Composite

Part:BBa_K3570001:Design

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-22)


Sweet rose taste induction system in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4894
    Illegal BamHI site found at 3752
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
    Illegal AgeI site found at 1551
    Illegal AgeI site found at 4507
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

YPRcΔ15 up/down - S. cerevisiae genome; Geraniol synthase - Catharanthus roseus genome; Brazzein - Madagascar periwinkle genome; Gal 1/10 promoter - S. cerevisiae genome; CYC1 terminator - S. cerevisiae genome; PGK terminator - S. cerevisiae genome; LEU2 selective locus - S. cerevisiae genome;

References